Fig 1: The effect of estradiol on antimicrobial peptide release. Beta-defensin 1 (A), LL-37 (B), RNase7 (C) and Beta-defensin 2 (D) release from Cas9 or NLRP3-deficient (NLRP3 gRNA) bladder epithelial cells stimulated with estradiol (10 nM) for 24 h. Data are presented as mean ± SEM of four independent experiments. Asterisks show statistical significance compared to respective unstimulated control (** p < 0.01, *** p < 0.001).